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human mirna onearray ® 4.1 microarray chips  (Phalanx Biotech)

 
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    Phalanx Biotech human mirna onearray ® 4.1 microarray chips
    Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.
    Human Mirna Onearray ® 4.1 Microarray Chips, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+onearray+%C2%AE+4%2E1+microarray+chips/human+mirna+onearray+v4/pmc11432929-185-30-37
    Average 90 stars, based on 1 article reviews
    human mirna onearray ® 4.1 microarray chips - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing"

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms251810125

    Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.
    Figure Legend Snippet: Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.

    Techniques Used:

    Differential expression of miRNAs in HaCaT cells under high-glucose/hypoxia versus normal conditions. ( A ) Heat map and cluster dendrogram of 143 differentially expressed miRNAs. Columns represent miRNA expression at 24 h and 48 h under normal (24 hr1 and 48 hr1) and high-glucose/hypoxia (24 hr4 and 48 hr4) conditions. ( B ) Heat map and cluster dendrogram of 11 miRNAs with the highest changes on the expression levels at 24 and 48 h post-wounding (labeled with red asterisks in ( A )). This heat map shows miRNA changes after the normalization of the high-glucose/hypoxia condition over the normal condition. Subsequent qRT-PCR verification on these miRNAs is shown in .
    Figure Legend Snippet: Differential expression of miRNAs in HaCaT cells under high-glucose/hypoxia versus normal conditions. ( A ) Heat map and cluster dendrogram of 143 differentially expressed miRNAs. Columns represent miRNA expression at 24 h and 48 h under normal (24 hr1 and 48 hr1) and high-glucose/hypoxia (24 hr4 and 48 hr4) conditions. ( B ) Heat map and cluster dendrogram of 11 miRNAs with the highest changes on the expression levels at 24 and 48 h post-wounding (labeled with red asterisks in ( A )). This heat map shows miRNA changes after the normalization of the high-glucose/hypoxia condition over the normal condition. Subsequent qRT-PCR verification on these miRNAs is shown in .

    Techniques Used: Quantitative Proteomics, Expressing, Labeling, Quantitative RT-PCR

    Verification of the expression patterns of the cDNA microarray by qRT-PCR. ( A ) Differentially expressed miRNAs at 24 h post-wounding. ( B ) Differentially expressed miRNAs at 48 h post-wounding. A total of 11 miRNAs were selected for further verification by qRT-PCR in this study. In the figures, the relative miRNA expression levels are all expressed on a log2 scale, with data from the cDNA microarray shown in blue, and data from three independent experiments of qRT-PCR shown in red.
    Figure Legend Snippet: Verification of the expression patterns of the cDNA microarray by qRT-PCR. ( A ) Differentially expressed miRNAs at 24 h post-wounding. ( B ) Differentially expressed miRNAs at 48 h post-wounding. A total of 11 miRNAs were selected for further verification by qRT-PCR in this study. In the figures, the relative miRNA expression levels are all expressed on a log2 scale, with data from the cDNA microarray shown in blue, and data from three independent experiments of qRT-PCR shown in red.

    Techniques Used: Expressing, Microarray, Quantitative RT-PCR

    Effects of miR-3138 mimic and miR-3679-5p inhibitor transfection on keratinocyte migration. The present experiments were performed under normal ( A – C ) and high-glucose/hypoxic conditions ( D – F ). ( A , D ) Relative miR-3138 and miR-3679-5p levels post-transfection. ( B , E ) Representative images of keratinocyte migration after the transfection with miRNA mimic and inhibitor. ( C , F ) Average wound closure percentages from three independent experiments after the transfection with miRNA mimic and inhibitor. Different letters labeled at the top of columns indicate statistically significant differences ( p < 0.05).
    Figure Legend Snippet: Effects of miR-3138 mimic and miR-3679-5p inhibitor transfection on keratinocyte migration. The present experiments were performed under normal ( A – C ) and high-glucose/hypoxic conditions ( D – F ). ( A , D ) Relative miR-3138 and miR-3679-5p levels post-transfection. ( B , E ) Representative images of keratinocyte migration after the transfection with miRNA mimic and inhibitor. ( C , F ) Average wound closure percentages from three independent experiments after the transfection with miRNA mimic and inhibitor. Different letters labeled at the top of columns indicate statistically significant differences ( p < 0.05).

    Techniques Used: Transfection, Migration, Labeling

    Bioinformatic prediction of the target genes for miR-3138 ( A ) and miR-3679-5p ( B ). Venn diagrams show overlapped target numbers predicted by TargetScan (red), miRDB (green), and DIANA (blue) databases. The pairing patterns between the sequences of target gene 3’UTR and miRNA are shown below each diagram.
    Figure Legend Snippet: Bioinformatic prediction of the target genes for miR-3138 ( A ) and miR-3679-5p ( B ). Venn diagrams show overlapped target numbers predicted by TargetScan (red), miRDB (green), and DIANA (blue) databases. The pairing patterns between the sequences of target gene 3’UTR and miRNA are shown below each diagram.

    Techniques Used:

    Primers used in this study.
    Figure Legend Snippet: Primers used in this study.

    Techniques Used: Sequencing



    Similar Products

    human mirna onearray ® 4.1 microarray chips  (Phalanx Biotech)
    90
    Phalanx Biotech human mirna onearray ® 4.1 microarray chips
    Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.
    Human Mirna Onearray ® 4.1 Microarray Chips, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+onearray+%C2%AE+4%2E1+microarray+chips/human+mirna+onearray+v4/pmc11432929-185-30-37
    Average 90 stars, based on 1 article reviews
    human mirna onearray ® 4.1 microarray chips - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    doi: 10.3390/ijms251810125

    Figure Lengend Snippet: Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.

    Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

    Techniques:

    Differential expression of miRNAs in HaCaT cells under high-glucose/hypoxia versus normal conditions. ( A ) Heat map and cluster dendrogram of 143 differentially expressed miRNAs. Columns represent miRNA expression at 24 h and 48 h under normal (24 hr1 and 48 hr1) and high-glucose/hypoxia (24 hr4 and 48 hr4) conditions. ( B ) Heat map and cluster dendrogram of 11 miRNAs with the highest changes on the expression levels at 24 and 48 h post-wounding (labeled with red asterisks in ( A )). This heat map shows miRNA changes after the normalization of the high-glucose/hypoxia condition over the normal condition. Subsequent qRT-PCR verification on these miRNAs is shown in .

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    doi: 10.3390/ijms251810125

    Figure Lengend Snippet: Differential expression of miRNAs in HaCaT cells under high-glucose/hypoxia versus normal conditions. ( A ) Heat map and cluster dendrogram of 143 differentially expressed miRNAs. Columns represent miRNA expression at 24 h and 48 h under normal (24 hr1 and 48 hr1) and high-glucose/hypoxia (24 hr4 and 48 hr4) conditions. ( B ) Heat map and cluster dendrogram of 11 miRNAs with the highest changes on the expression levels at 24 and 48 h post-wounding (labeled with red asterisks in ( A )). This heat map shows miRNA changes after the normalization of the high-glucose/hypoxia condition over the normal condition. Subsequent qRT-PCR verification on these miRNAs is shown in .

    Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

    Techniques: Quantitative Proteomics, Expressing, Labeling, Quantitative RT-PCR

    Verification of the expression patterns of the cDNA microarray by qRT-PCR. ( A ) Differentially expressed miRNAs at 24 h post-wounding. ( B ) Differentially expressed miRNAs at 48 h post-wounding. A total of 11 miRNAs were selected for further verification by qRT-PCR in this study. In the figures, the relative miRNA expression levels are all expressed on a log2 scale, with data from the cDNA microarray shown in blue, and data from three independent experiments of qRT-PCR shown in red.

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    doi: 10.3390/ijms251810125

    Figure Lengend Snippet: Verification of the expression patterns of the cDNA microarray by qRT-PCR. ( A ) Differentially expressed miRNAs at 24 h post-wounding. ( B ) Differentially expressed miRNAs at 48 h post-wounding. A total of 11 miRNAs were selected for further verification by qRT-PCR in this study. In the figures, the relative miRNA expression levels are all expressed on a log2 scale, with data from the cDNA microarray shown in blue, and data from three independent experiments of qRT-PCR shown in red.

    Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

    Techniques: Expressing, Microarray, Quantitative RT-PCR

    Effects of miR-3138 mimic and miR-3679-5p inhibitor transfection on keratinocyte migration. The present experiments were performed under normal ( A – C ) and high-glucose/hypoxic conditions ( D – F ). ( A , D ) Relative miR-3138 and miR-3679-5p levels post-transfection. ( B , E ) Representative images of keratinocyte migration after the transfection with miRNA mimic and inhibitor. ( C , F ) Average wound closure percentages from three independent experiments after the transfection with miRNA mimic and inhibitor. Different letters labeled at the top of columns indicate statistically significant differences ( p < 0.05).

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    doi: 10.3390/ijms251810125

    Figure Lengend Snippet: Effects of miR-3138 mimic and miR-3679-5p inhibitor transfection on keratinocyte migration. The present experiments were performed under normal ( A – C ) and high-glucose/hypoxic conditions ( D – F ). ( A , D ) Relative miR-3138 and miR-3679-5p levels post-transfection. ( B , E ) Representative images of keratinocyte migration after the transfection with miRNA mimic and inhibitor. ( C , F ) Average wound closure percentages from three independent experiments after the transfection with miRNA mimic and inhibitor. Different letters labeled at the top of columns indicate statistically significant differences ( p < 0.05).

    Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

    Techniques: Transfection, Migration, Labeling

    Bioinformatic prediction of the target genes for miR-3138 ( A ) and miR-3679-5p ( B ). Venn diagrams show overlapped target numbers predicted by TargetScan (red), miRDB (green), and DIANA (blue) databases. The pairing patterns between the sequences of target gene 3’UTR and miRNA are shown below each diagram.

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    doi: 10.3390/ijms251810125

    Figure Lengend Snippet: Bioinformatic prediction of the target genes for miR-3138 ( A ) and miR-3679-5p ( B ). Venn diagrams show overlapped target numbers predicted by TargetScan (red), miRDB (green), and DIANA (blue) databases. The pairing patterns between the sequences of target gene 3’UTR and miRNA are shown below each diagram.

    Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

    Techniques:

    Primers used in this study.

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

    doi: 10.3390/ijms251810125

    Figure Lengend Snippet: Primers used in this study.

    Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

    Techniques: Sequencing